Step 1

Install and Setup

Please review the detailed system requirements and install instructions available here. If you are currently running v0, you can seamlessly install and run v2 alongside, as they use different port numbers. These cover workstation and cluster installs, file configuration and how to update cryoSPARC v2 when new versions become available. For assistance, please refer to the cryoSPARC discussion forum or send us an email at feedback@structura.bio.

Step 2

T20S Tutorial

We recommend starting off with the T20S Tutorial to become familiar with cryoSPARC v2. The tutorial takes you from raw movies through to a refined structure, using a small set of 20 movies.

Step 3

Process Your Cryo-EM Data

The Documentation section of this website is a live reference for all things cryoSPARC v2 and is continuously updated with new information and links.

  • The Basics: Consult this section for a quick walk-through of everything you need to know about processing data in cryoSPARC v2, including:
    • How to organize projects and workspaces
    • Navigation, keyboard shortcuts and the tree view
    • How to connect inputs and queue jobs and modify parameters
    • Killing, deleting, coning, moving and linking jobs
    • How to use the Resource Manager
    • How to set up automated workflows that run on their own
  • Jobs and Parameters: The Guide to Jobs and Common Parameters contains comprehensive information on all job types in cryoSPARC v2, with detailed information on:
    • What these jobs do, and when to use them
    • Inputs and outputs
    • Commonly adjusted parameters
    • Common next steps
  • Tutorials: Find in-depth tutorials covering various aspects of processing data in cryoSPARC. We are always working to add more.
  • Blog: On the blog, you can find data processing case studies, information on new features and fixes, new papers published, and cryo-EM related news.
  • Discussion Forum: Contribute to and learn from a vibrant community of cryoSPARC users who may have similar questions on the discussion forum.
  • Stay in the know: You can sign up for our newsletter to receive updates on new features and data processing tips.
  • Contact Us: Issues or feedback? Drop us a line at feedback@structura.bio, we would love to hear from you!

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