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CryoSPARC v4.5 Now Available

Featuring a new micrograph denoiser, automatic 2D/3D class selection, local file upload and more!

Unlock the potential of cryo-EM with CryoSPARC

CryoSPARC is a state of the art scientific software platform for cryo-electron microscopy (cryo-EM) used in research and drug discovery pipelines.

CrySPARC reconstruction


Extract valuable insight from single particle cryo-EM data

CryoSPARC combines powerful innovations in 3D reconstruction algorithms with specially designed software to provide a streamlined end-to-end single particle cryo-EM workflow. Rapidly solve high-resolution structures of biologically important targets, with advanced tools for membrane proteins, heterogeneous samples, and flexible molecules. Process 3D refinements in minutes on GPU.

Read more about the scientific advances in CryoSPARC in Nature Methods, check out hundreds of studies that have used CryoSPARC, and subscribe to our newsletter to stay up to date.

Software System

CryoSPARC is a globally trusted complete solution for cryo-EM data processing

  • Ultra-fast end-to-end processing of raw cryo-EM data and reconstruction of electron density maps, ready for ingestion into model building software
  • Optimized algorithms and GPU acceleration at all stages, from pre-processing through particle picking, 2D particle classification, 3D ab-initio structure determination, high resolution refinement, and heterogeneity analysis
  • Specialized and unique tools for therapeutically relevant targets, membrane proteins, continuously flexible structures
  • Interactive, visual and iterative experimentation for even the most complex workflows

CryoSPARC Live

Keep pace with data collection

CryoSPARC Live™ is an extension of the CryoSPARC platform that enables real-time cryo-EM data quality assessment and decision making during live data collection, as well as an expedited, streamlined workflow for processing already available data.

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crySPARC Live user interface


CryoSPARC™ and CryoSPARC Live™ are available free of charge for non-profit academic use. Please request a license here. Commercial/for-profit licensing inquiries can be sent to

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CryoSPARC is developed and supported by Structura Biotechnology Inc. Please contact to find out how CryoSPARC can add value to your cryo-EM workflow.

About Structura Biotechnology

Structura builds software that enables fast, accurate and automated cryo-EM for drug discovery and research. We work closely with pharmaceutical companies and research labs globally. Learn more →