Introducing 3D Flexible RefinementIntroducing 3D Flexible Refinement Learn more
View our new, comprehensive cryoSPARC Guide, available at guide.cryosparc.com. This new site will consolidate all existing documentation and is being populated with more cryo-EM data processing tutorials, videos, tips, descriptions of methods and case studies.
Quickstart
If you are new to cryoSPARC, we recommend working through the Introductory Tutorial
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Reference Guides
Verify cryoSPARC installation with the Extensive Workflow (Sysadmin Guide)
Installation, Setup and User Management
User Management
Installation, Setup and User Management
Introductory Tutorial (T20S)
Introduction to cryoSPARC
Job Builder
Introduction to cryoSPARC
Case Study: Processing EMPIAR-10291 (300 Micrographs) to 3.4Å in 1 hour 25 minutes
Introduction to cryoSPARC
Accessing the cryoSPARC User Interface Remotely
Introduction to cryoSPARC
Negative Stain
Data Processing → Files, Data and Import
Phase Plate Data
Data Processing → Files, Data and Import
EER File Support
Data Processing → Files, Data and Import
Patch Motion and Patch CTF
Data Processing → Preprocessing
Manually Curate Exposures
Data Processing → Preprocessing
Particle Picking Calibration
Data Processing → Particle Picking
Topaz in cryoSPARC
Data Processing → Particle Picking
Deep Particle Picking
Data Processing → Particle Picking
Helical Refinement Case Study: EMPIAR-10031 (MAVS)
Data Processing → Helical Reconstruction
CTF Refinement
Data Processing → 3D Refinement
Local Refinement
Data Processing → Local Refinement
Local Refinement Case Study (Yeast U4/U6.U5 tri-snRNP)
Data Processing → Local Refinement
3D Variability Analysis: Part One
Data Processing → Variability Analysis
3D Variability Analysis: Part Two
Data Processing → Variability Analysis
Queuing Directly to a GPU
Software and Data Management → Software Utility Features
SSD Particle Caching in cryoSPARC
Software and Data Management → Software Utility Features
Job Priority Queuing
Software and Data Management → Software Utility Features
Data Management
Software and Data Management → Data Management, Import and Export
CryoSPARC Live Session Data Management
Software and Data Management → Data Management, Import and Export
Manipulating .cs Files Created by CryoSPARC
Software and Data Management → Data Management, Import and Export
Migrating Your cryoSPARC Instance
Software and Data Management → Data Management, Import and Export
Mask Selection and Generation in UCSF Chimera
Tips and Tricks