Note

This version replaces CryoSPARC v4.5.2, released on June 3, 2024.

New

Check for Corrupt Micrographs job tests for and separates micrograph files that cannot be successfully read from disk.

Fixed

Refinement jobs default parameters have been changed so that the jobs use the half-set split from the input particles by default. This ensures that repeated application of Reference Based Motion Correction and refinement does not cause mixing of the half-sets. The Particle Sets Tool job also now has an option to balance unequal half-set splits.

Fixed

Orientation Diagnostics no longer fails on CPU-only worker nodes, and requests the correct number of CPUs when launching on a cluster.

Fixed

Relative signal plots in Orientation Diagnostics jobs are now less crowded.

Fixed

The Topaz Extract job now allows input in units of estimated particle diameter for the radius of extracted region parameter.

Fixed

Import Result Group no longer fails to import exposures when some paths are missing.

Fixed

Regroup 3D no longer outputs incorrect particles when classes are manually excluded.

Fixed

Fixed an issue where for some large CryoSPARC Live sessions, scrolling through the exposure feed to earlier exposures could sometimes cause the application server to crash with a memory leak.

Fixed

Excess UserWarnings are no longer printed in job log output.

Fixed

The default value for CRYOSPARC_JOB_LAUNCH_TIMEOUT is now extended to 60 seconds.