Digging deeperDigging Deeper: Particle Curation using Decoy ClassificationParticle cleanup does not always have to happen in 2D. Decoy classification uses Heterogeneous Refinement in CryoSPARC to curate particles directly in 3D, providing a flexible strategy for separating junk, preserving underrepresented views, and distinguishing different particle populations.#cryo-em#cryosparc#methods#structural-biology#workflowCryoSPARC Team·September 23, 2026
Digging deeperDigging deeper: From micrograph curation to efficient particle pickingDigging into Sections 1 to 4 of one of our CryoSPARC case studies, we explore strategies for streamlining preprocessing and particle curation, using tools such as Micrograph Junk Detector and Micrograph Denoiser to efficiently move from initial micrographs to a curated particle set.#case-study#cryo-em#cryosparc#methods#structural-biology#workflowCryoSPARC Team·September 10, 2026
Digging deeperDigging Deeper: Why refinements can be robust to the presence of junk particlesThe effect of parameter settings on 3D refinement: Minimize over per-particle scale, Adaptive marginalization, Non-uniform regularization, and Masking#cryosparc#cryo-em#refinement#methods#workflow#case-studyCryoSPARC Team·July 21, 2026
