Automated WorkflowsOvercoming Preferred Orientation with Automated Workflows in CryoSPARC™Preferred orientation is a persistent challenge in cryo-EM, but what can be done during data processing? Explore how advanced automated workflows in CryoSPARC can mitigate the effects of preferred orientation, improving map quality and interpretability across nine challenging datasets.#automated-workflows#cryo-em#cryosparc#sbdd#structural-biologyCryoSPARC Team·October 28, 2026
DiscoveriesCryo-EM Insights into pH Regulation in Breast CancerCryo-EM and computational modeling reveal the structure and transport mechanism of NBCn1, a key pH regulator in breast cancer.#cryo-em#cryosparc#discoveries#structural-biologyCryoSPARC Team·October 8, 2026
Digging deeperDigging Deeper: Particle Curation using Decoy ClassificationParticle cleanup does not always have to happen in 2D. Decoy classification uses Heterogeneous Refinement in CryoSPARC to curate particles directly in 3D, providing a flexible strategy for separating junk, preserving underrepresented views, and distinguishing different particle populations.#cryo-em#cryosparc#methods#structural-biology#workflowCryoSPARC Team·September 23, 2026
Digging deeperDigging deeper: From micrograph curation to efficient particle pickingDigging into Sections 1 to 4 of one of our CryoSPARC case studies, we explore strategies for streamlining preprocessing and particle curation, using tools such as Micrograph Junk Detector and Micrograph Denoiser to efficiently move from initial micrographs to a curated particle set.#case-study#cryo-em#cryosparc#methods#structural-biology#workflowCryoSPARC Team·September 10, 2026
DiscoveriesThe Pain Paradox: Activating TRPV1 to Silence Its ResponseCombining rational drug design, functional studies, and cryo-EM, researchers characterize a selective TRPV1 agonist that promotes desensitization and prolonged pain relief.#structural-biology#cryosparc#cryo-em#discoveries#sbddCryoSPARC Team·September 1, 2026
CryoSPARC UpdatesInstance Recovery in CryoSPARC v5Discover the Instance Recovery feature introduced in CryoSPARC v5: how to recover a CryoSPARC instance if the database directory is corrupted or lost.#it-management#cryo-em#cryosparc#overview#releaseCryoSPARC Team·August 27, 2026
DiscoveriesMalaria Invasion at Molecular Resolution: From Protein Inhibitors to Membrane RemodelingUsing single-particle cryo-EM, researchers revealed how the malaria parasite's moving junction remodels host membranes and used its native structure to guide the design of a protein inhibitor of parasite invasion.#structural-biology#cryosparc#cryo-em#discoveries#sbddCryoSPARC Team·August 20, 2026
Case StudiesMixing Two Cryo-EM Datasets on Purpose: A Cryo-EM Heterogeneity ChallengeUsing a controlled FaNaC1 dataset, we compared 3D Classification, Heterogeneous Refinement, 3D Variability Analysis, and 3D Flexibility Analysis to explore practical strategies for resolving both discrete and continuous heterogeneity in cryo-EM.#structural-biology#cryosparc#cryo-em#heterogeneity#case-studyCryoSPARC Team·August 13, 2026
DiscoveriesCryo-EM Reveals the Molecular Machinery of Tubulin AssemblyBy capturing transient β-tubulin-bound intermediates with cryo-EM, researchers reconstructed the molecular mechanism of α/β-tubulin biogenesis, explaining how mutations in the assembly machinery lead to tubulin biogenesis disorders.#structural-biology#cryosparc#cryo-em#discoveriesCryoSPARC Team·July 30, 2026
Digging deeperDigging Deeper: Why refinements can be robust to the presence of junk particlesThe effect of parameter settings on 3D refinement: Minimize over per-particle scale, Adaptive marginalization, Non-uniform regularization, and Masking#cryosparc#cryo-em#refinement#methods#workflow#case-studyCryoSPARC Team·July 21, 2026
DiscoveriesMind The Gap: Cryo-EM Reveals Lipids Intercalating Connexin AssembliesUsing single particle cryo-EM on plaque-mimicking nanodiscs, researchers determined multiple connexin assembly states and uncovered lipid-protein interactions that may help understand gap junction organization and function.#structural-biology#cryosparc#cryo-em#discoveriesCryoSPARC Team·July 8, 2026
Automated WorkflowsAutomated Workflows in CryoSPARC™How to make use of one-click, automated data processing workflows that can provide equal or better results as manual processing#structural-biology#cryosparc#cryo-em#automated-workflows#gpcrCryoSPARC Team·June 25, 2026
DiscoveriesScratching Beneath the Surface: Cryo-EM Guides New Opioid Drug DesignCryo-EM revealed the molecular basis of KOR signaling bias, enabling structure-based drug design of an anti-pruritic peptide with reduced side effects.#structural-biology#cryosparc#cryo-em#discoveries#sbdd#gpcrCryoSPARC Team·June 16, 2026
Case StudiesA Complex Story: A Case Study in Cryo-EM HeterogeneityFrom active and deactive states to additional respiratory complexes, this case study shows how a single cryo-EM dataset can tell many different stories when viewed through the lens of heterogeneity.#structural-biology#cryosparc#cryo-em#heterogeneity#case-studyCryoSPARC Team·June 9, 2026
DiscoveriesA New Step in the Fight Against MeaslesHow Cryo-EM allows a closer look at the Human neutralizing antibodies targeting the measles virus#structural-biology#cryosparc#discoveries#cryo-emCryoSPARC Team·June 5, 2026
Small Protein TargetsThe Resolution Revolution Is Still OngoingHigh-Resolution Ab-Initio Reconstruction: Extending Cryo-EM to Smaller Particles with CryoSPARC™#structural-biology#cryosparc#cryo-em#small-proteinCryoSPARC Team·May 27, 2026
CryoSPARC UpdatesCryoSPARC v5.0 stable releaseExplore the new features, expanded capabilities, and a more intuitive processing experience!#structural-biology#cryosparc#release#overview#cryo-emCryoSPARC Team·May 27, 2026
