#cryo-em

15 posts with this tag.

Digging Deeper: Particle Curation using Decoy Classification

Particle cleanup does not always have to happen in 2D. Decoy classification uses Heterogeneous Refinement in CryoSPARC to curate particles directly in 3D, providing a flexible strategy for separating junk, preserving underrepresented views, and distinguishing different particle populations.

#cryo-em #cryosparc #methods #structural-biology #workflow

CryoSPARC Team

·September 23, 2026

micrograph-denoiser-blob-picker-micrograph-junk-detector

Digging deeper

Digging deeper: From micrograph curation to efficient particle picking

Digging into Sections 1 to 4 of one of our CryoSPARC case studies, we explore strategies for streamlining preprocessing and particle curation, using tools such as Micrograph Junk Detector and Micrograph Denoiser to efficiently move from initial micrographs to a curated particle set.

#case-study #cryo-em #cryosparc #methods #structural-biology #workflow

CryoSPARC Team

·September 10, 2026

Discoveries

The Pain Paradox: Activating TRPV1 to Silence Its Response

Combining rational drug design, functional studies, and cryo-EM, researchers characterize a selective TRPV1 agonist that promotes desensitization and prolonged pain relief.

#structural-biology #cryosparc #cryo-em #discoveries #sbdd

CryoSPARC Team

·September 1, 2026

CryoSPARC Updates

Instance Recovery in CryoSPARC v5

Discover the Instance Recovery feature introduced in CryoSPARC v5: how to recover a CryoSPARC instance if the database directory is corrupted or lost.

#it-management #cryo-em #cryosparc #overview #release

CryoSPARC Team

·August 27, 2026

Discoveries

Malaria Invasion at Molecular Resolution: From Protein Inhibitors to Membrane Remodeling

Using single-particle cryo-EM, researchers revealed how the malaria parasite's moving junction remodels host membranes and used its native structure to guide the design of a protein inhibitor of parasite invasion.

#structural-biology #cryosparc #cryo-em #discoveries #sbdd

CryoSPARC Team

·August 20, 2026

Case Studies

Mixing Two Cryo-EM Datasets on Purpose: A Cryo-EM Heterogeneity Challenge

Using a controlled FaNaC1 dataset, we compared 3D Classification, Heterogeneous Refinement, 3D Variability Analysis, and 3D Flexibility Analysis to explore practical strategies for resolving both discrete and continuous heterogeneity in cryo-EM.

#structural-biology #cryosparc #cryo-em #heterogeneity #case-study

CryoSPARC Team

·August 13, 2026

Discoveries

Cryo-EM Reveals the Molecular Machinery of Tubulin Assembly

By capturing transient β-tubulin-bound intermediates with cryo-EM, researchers reconstructed the molecular mechanism of α/β-tubulin biogenesis, explaining how mutations in the assembly machinery lead to tubulin biogenesis disorders.

#structural-biology #cryosparc #cryo-em #discoveries

CryoSPARC Team

·July 30, 2026

Digging deeper

Digging Deeper: Why refinements can be robust to the presence of junk particles

The effect of parameter settings on 3D refinement: Minimize over per-particle scale, Adaptive marginalization, Non-uniform regularization, and Masking

#cryosparc #cryo-em #refinement #methods #workflow #case-study

CryoSPARC Team

·July 21, 2026

Discoveries

Mind The Gap: Cryo-EM Reveals Lipids Intercalating Connexin Assemblies

Using single particle cryo-EM on plaque-mimicking nanodiscs, researchers determined multiple connexin assembly states and uncovered lipid-protein interactions that may help understand gap junction organization and function.

#structural-biology #cryosparc #cryo-em #discoveries

CryoSPARC Team

·July 8, 2026

Automated Workflows

Automated Workflows in CryoSPARC™

How to make use of one-click, automated data processing workflows that can provide equal or better results as manual processing

#structural-biology #cryosparc #cryo-em #automated-workflows #gpcr

CryoSPARC Team

·June 25, 2026

Discoveries

Scratching Beneath the Surface: Cryo-EM Guides New Opioid Drug Design

Cryo-EM revealed the molecular basis of KOR signaling bias, enabling structure-based drug design of an anti-pruritic peptide with reduced side effects.

#structural-biology #cryosparc #cryo-em #discoveries #sbdd #gpcr

CryoSPARC Team

·June 16, 2026

Case Studies

A Complex Story: A Case Study in Cryo-EM Heterogeneity

From active and deactive states to additional respiratory complexes, this case study shows how a single cryo-EM dataset can tell many different stories when viewed through the lens of heterogeneity.

#structural-biology #cryosparc #cryo-em #heterogeneity #case-study

CryoSPARC Team

·June 9, 2026