Case StudiesMixing Two Cryo-EM Datasets on Purpose: A Cryo-EM Heterogeneity ChallengeUsing a controlled FaNaC1 dataset, we compared 3D Classification, Heterogeneous Refinement, 3D Variability Analysis, and 3D Flexibility Analysis to explore practical strategies for resolving both discrete and continuous heterogeneity in cryo-EM.#structural-biology#cryosparc#cryo-em#heterogeneity#case-studyCryoSPARC Team·August 13, 2026
Digging deeperDigging Deeper: Why refinements can be robust to the presence of junk particlesThe effect of parameter settings on 3D refinement: Minimize over per-particle scale, Adaptive marginalization, Non-uniform regularization, and Masking#cryosparc#cryo-em#refinement#methods#workflow#case-studyCryoSPARC Team·July 21, 2026
Case StudiesA Complex Story: A Case Study in Cryo-EM HeterogeneityFrom active and deactive states to additional respiratory complexes, this case study shows how a single cryo-EM dataset can tell many different stories when viewed through the lens of heterogeneity.#structural-biology#cryosparc#cryo-em#heterogeneity#case-studyCryoSPARC Team·June 9, 2026
