Digging deeperDigging deeper: From micrograph curation to efficient particle pickingDigging into Sections 1 to 4 of one of our CryoSPARC case studies, we explore strategies for streamlining preprocessing and particle curation, using tools such as Micrograph Junk Detector and Micrograph Denoiser to efficiently move from initial micrographs to a curated particle set.#case-study#cryo-em#cryosparc#methods#structural-biology#workflowCryoSPARC Team·September 10, 2026
Case StudiesMixing Two Cryo-EM Datasets on Purpose: A Cryo-EM Heterogeneity ChallengeUsing a controlled FaNaC1 dataset, we compared 3D Classification, Heterogeneous Refinement, 3D Variability Analysis, and 3D Flexibility Analysis to explore practical strategies for resolving both discrete and continuous heterogeneity in cryo-EM.#structural-biology#cryosparc#cryo-em#heterogeneity#case-studyCryoSPARC Team·August 13, 2026
Digging deeperDigging Deeper: Why refinements can be robust to the presence of junk particlesThe effect of parameter settings on 3D refinement: Minimize over per-particle scale, Adaptive marginalization, Non-uniform regularization, and Masking#cryosparc#cryo-em#refinement#methods#workflow#case-studyCryoSPARC Team·July 21, 2026
Case StudiesA Complex Story: A Case Study in Cryo-EM HeterogeneityFrom active and deactive states to additional respiratory complexes, this case study shows how a single cryo-EM dataset can tell many different stories when viewed through the lens of heterogeneity.#structural-biology#cryosparc#cryo-em#heterogeneity#case-studyCryoSPARC Team·June 9, 2026
